Cadmium and chlorpyrifos inhibit cellular immune response in spleen of rats

Cadmium (Cd) and chlorpyrifos (CPF) are common pollutants coexisting in the environment, and both of them have been reported to have immunotoxicity to organisms. However, the joint effects of these two chemicals on the immune system are still unknown. In this study, we used CdCl₂ and CPF to study their combined effects on immune functions in the spleen of rats. In in vivo experiments, SD rats were exposed to different doses of CdCl₂ (0.7 and 6 mg kg^-1 body weight/day) and CPF (1.7 and 15 mg kg^-1 body weight/day) or their combinations for consecutive 28 days. The proliferation and cytokine production ability of the splenocytes isolated from the treated animals were assessed. In in vitro experiments, we used different concentrations of CdCl₂ and CPF to treat concanavalin A (Con A)-induced splenocytes isolated from untreated rats. We found that the combination of CPF and high dose of CdCl₂ had a synergistic inhibitory effect on production of IFN-γ by spleen cells induced by Con A. The in vitro results showed that two chemicals had different effects on the cell proliferation and cytokine production depending on the exposure doses and time. This result suggests that exposure to both CdCl₂ and CPF at the environmentally-relevant low dose may be potentially more hazardous than exposure to each individual toxicant.