Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Carcinogenicity of butyl benzyl phthalate, dibutyl phthalate, and diisononyl phthalate.

The Lancet. Oncology·2026
Same author

Changes in HDAC Expression and Activity by Oolongtheanin Digallate and Theasinensins and the Onset of Apoptosis.

Molecules (Basel, Switzerland)·2026
Same author

Chemical, Biological, and Ecological Evidence for Aerobic Deoxynivalenol Detoxification in Agronomic Soil-Derived Bacterial Communities.

Toxins·2026
Same author

NAMs for closing knowledge gaps in assessment of respiratory uptake - a PARC project.

Frontiers in toxicology·2026
Same author

Beyond quantitative indicators: Comprehensive characterization of indoor fungal contamination in Portuguese elementary schools.

Environmental research·2026
Same author

Using effect biomarker thresholds in regulatory risk assessment: an OECD-based framework for assessing cumulative exposures to chemical mixtures.

Environment international·2026

Related Experiment Video

Updated: Mar 6, 2026

Dried Blood and Serum Spots As A Useful Tool for Sample Storage to Evaluate Cancer Biomarkers
08:44

Dried Blood and Serum Spots As A Useful Tool for Sample Storage to Evaluate Cancer Biomarkers

Published on: June 11, 2018

11.0K

Multi-mycotoxin analysis using dried blood spots and dried serum spots.

Bernd Osteresch1, Susana Viegas2,3, Benedikt Cramer1

  • 1Institute of Food Chemistry, Westfälische Wilhelms-Universität Münster, Corrensstr. 45, 48149, Münster, Germany.

Analytical and Bioanalytical Chemistry
|March 17, 2017
PubMed
Summary

A new method quantifies 27 mycotoxins in human blood using HPLC-MS/MS on dried blood spots. This biomonitoring approach detected ochratoxins and enniatin B in a German cohort, aiding human exposure assessment.

Keywords:
BiomonitoringDried blood spotDried serum spotHPLC-MS/MSMass spectrometryMycotoxin

More Related Videos

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques
11:28

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Published on: March 13, 2015

41.7K
Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS
08:38

Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS

Published on: November 8, 2015

17.5K

Related Experiment Videos

Last Updated: Mar 6, 2026

Dried Blood and Serum Spots As A Useful Tool for Sample Storage to Evaluate Cancer Biomarkers
08:44

Dried Blood and Serum Spots As A Useful Tool for Sample Storage to Evaluate Cancer Biomarkers

Published on: June 11, 2018

11.0K
Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques
11:28

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Published on: March 13, 2015

41.7K
Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS
08:38

Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS

Published on: November 8, 2015

17.5K

Area of Science:

  • Environmental Health Sciences
  • Analytical Chemistry
  • Toxicology

Background:

  • Mycotoxins are toxic secondary metabolites produced by fungi, posing significant risks to human health through contaminated food and environmental exposure.
  • Accurate biomonitoring of mycotoxin exposure in humans is crucial for risk assessment and public health surveillance.
  • Existing methods for mycotoxin analysis in biological matrices can be complex and time-consuming.

Purpose of the Study:

  • To develop and validate a rapid, sensitive, and comprehensive multi-mycotoxin method for human biomonitoring.
  • To quantify 27 key mycotoxins and their metabolites in human serum and whole blood using dried sample matrices.
  • To assess the stability of mycotoxins in dried blood spots (DBS) and dried serum spots (DSS) under typical storage conditions.

Main Methods:

  • Development of a multi-mycotoxin approach utilizing High-Performance Liquid Chromatography coupled with tandem Mass Spectrometry (HPLC-MS/MS).
  • Analysis of 27 mycotoxins and metabolites in dried serum spots (DSS) and dried blood spots (DBS).
  • Validation of the method including matrix effects, limit of quantification (LOQ), and recovery rates using matrix-matched calibration.

Main Results:

  • The validated HPLC-MS/MS method achieved low pg/mL LOQs and excellent recovery rates for most analytes in both DSS and DBS.
  • Analyte stability was investigated, highlighting potential issues for certain mycotoxins during room temperature storage of dried samples.
  • Application to a German cohort (n=50) revealed positive findings for ochratoxin A (OTA), 2'R-OTA, and ubiquitously for enniatin B (EnB) in DBS samples.

Conclusions:

  • A robust and validated multi-mycotoxin method for biomonitoring 27 mycotoxins and metabolites in dried blood and serum spots has been established.
  • The method employs fast sample preparation and sensitive HPLC-MS/MS detection, suitable for large-scale human exposure studies.
  • The findings demonstrate the presence of specific mycotoxins in the German population, underscoring the utility of this assay for public health monitoring.