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Updated: Mar 6, 2026

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

Published on: September 27, 2016

A Lateral Flow Biosensor for the Detection of Single Nucleotide Polymorphisms.

Lingwen Zeng¹, Zhuo Xiao²

¹Institute of Environment and Safety, Wuhan Academy of Agricultural Sciences, 1 Qinglingzhangjiawan, Hongshan District, Wuhan, China. zeng6@yahoo.com.

Methods in Molecular Biology (Clifton, N.J.)

|March 17, 2017

Summary

A novel lateral flow biosensor (LFB) detects single nucleotide polymorphisms (SNPs) using a two-step process. This cost-effective method offers potential for point-of-care diagnostics and genetic disease detection.

Keywords:

Gold nanoparticle Isothermal amplification Lateral flow biosensor Nucleic acid biosensor Point-of-care test SNP genotype

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Last Updated: Mar 6, 2026

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Published on: September 27, 2016

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Area of Science:

Biotechnology
Molecular Diagnostics
Biosensor Technology

Background:

Single nucleotide polymorphisms (SNPs) are crucial biomarkers for genetic diseases and personalized medicine.
Conventional SNP detection methods often require complex equipment and are time-consuming.
There is a need for rapid, cost-effective, and accessible diagnostic tools for SNP analysis.

Purpose of the Study:

To introduce a novel lateral flow biosensor (LFB) for the efficient detection of single nucleotide polymorphisms (SNPs).
To develop a simple, robust, and cost-effective assay for nucleic acid detection suitable for point-of-care applications.

Main Methods:

The assay involves a two-step process: circular strand displacement reaction followed by lateral flow biosensor detection.
Step 1 utilizes T4 DNA ligase for SNP site recognition and strand displacement DNA polymerase for signal amplification at a constant temperature.
Step 2 employs a lateral flow biosensor for rapid detection of the reaction product.

Main Results:

The developed lateral flow biosensor (LFB) enables rapid and cost-effective detection of single nucleotide polymorphisms (SNPs).
The assay bypasses the need for complicated machinery, making it suitable for point-of-care diagnostics.
The method demonstrates robustness and simplicity in SNP detection.

Conclusions:

This lateral flow biosensor (LFB) method presents a promising approach for SNP detection.
It holds significant potential for applications in genetic disease screening, personalized medicine, and identifying cancer or drug-resistant mutations.