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Updated: Mar 6, 2026

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Developmental changes in trak-mediated mitochondrial transport in neurons.

Omar Loss1, F Anne Stephenson1

  • 1School of Pharmacy University College London, 29/39 Brunswick Square, London WC1N 1AX, United Kingdom.

Molecular and Cellular Neurosciences
|March 17, 2017
PubMed
Summary

TRAK1 and TRAK2 proteins regulate mitochondrial transport in neurons. Their functions become specialized in mature neurons, with TRAK1 for axons and TRAK2 for dendrites, a pattern conserved across neuronal types during development.

Keywords:
Axonal transportKinesinMitochondrial transportTRAKTrafficking kinesin binding protein

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Molecular Biology

Background:

  • Kinesin adaptor proteins TRAK1 and TRAK2 are crucial for mitochondrial transport in neurons.
  • TRAKs form complexes with Miro and O-linked N-acetylglucosamine transferase (OGT) for mitochondrial trafficking.
  • Previous research suggested TRAK1 and TRAK2 have distinct roles in axonal versus dendritic transport.

Purpose of the Study:

  • To investigate the conserved roles of TRAK1 and TRAK2 in axonal and dendritic mitochondrial transport.
  • To compare TRAK-mediated mitochondrial mobility in hippocampal and cortical neurons during maturation.
  • To determine if TRAK protein function is exclusive to specific neuronal compartments or conserved across development.

Main Methods:

  • Comparative study of TRAK-mediated mitochondrial mobility in vitro.
  • Utilized shRNA gene knockdown to assess protein function.
  • Examined both hippocampal and cortical neurons across different maturation stages.

Main Results:

  • In mature neurons, TRAK1 predominantly mediates axonal mitochondrial transport, while TRAK2 mediates dendritic transport.
  • In young, developing neurons, TRAK1 and TRAK2 contribute similarly to mitochondrial transport in both axons and dendrites.
  • These maturation-dependent roles are conserved in both hippocampal and cortical neuronal subtypes.

Conclusions:

  • Mitochondrial transport regulation by TRAK proteins is modulated by neuronal maturation.
  • TRAK1 and TRAK2 exhibit distinct, yet conserved, roles in axonal and dendritic transport that emerge during neuronal development.
  • Findings highlight conserved mechanisms of mitochondrial trafficking regulation across different neuronal populations and developmental stages.