[The HIF1a polymorphism is a diagnostic marker of ischemic stroke]
E V Kovalevа1, B M Doronin2, V V Morozov1
1Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia.
Aim:
To study a variant of the C1772T polymorphism of the hypoxia inducible factor-1a (HIF1a) gene as a molecular genetic predictor of increased risk of ischemic stroke.
Material And Methods:
The study included patients with ischemic stroke which was confirmed clinically and by MRI (n=1200), the control group consisted of 500 patients without cardiovascular disease. DNA was extracted from the venous blood using the phenol-chloroform method. Determination of alleles was performed using allele-specific RealTime PCR as well as PCR/RFLP diagnosis.
Results:
The carriage of one or two copies of the allele T significantly increases the risk of ischemic stroke (OR=1.603, p=0.01). At the same time, the minor allele was not associated with the prognosis of the disease outcome.
Conclusion:
The results allow us to consider the HIF1 C1772T polymorphism as a new molecular genetic predictor of ischemic stroke.
