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Geraldo Elias Miranda1,2,3, Rodolfo Francisco Haltenhoff Melani3, Luiz Francisquini1
1Department of Social Dentistry, Piracicaba Dental School, UNICAMP - Universidade Estadual de Campinas, Piracicaba, SP, Brazil.
Brazilian Dental Journal
|March 17, 2017
Summary
Forensic experts can better detect fragmented teeth and bone using a 455 nm wavelength and orange filter. Dental root exhibits the highest fluorescence, aiding in biological material identification.
Area of Science:
- Forensic Science
- Biophotonics
- Dental Forensics
Background:
- Accurate identification of fragmented biological materials like teeth and bone is crucial in forensic investigations.
- Alternate Light Sources (ALS) offer a non-destructive method for detecting biological evidence.
Purpose of the Study:
- To determine the optimal wavelength and filter combination for detecting tooth and bone using ALS.
- To compare the fluorescence intensity of different dental and bone tissues.
Main Methods:
- Tooth and bone samples were illuminated with an ALS and photographed.
- Image analysis was performed using Adobe Photoshop™ and ImageJ™ software.
- Statistical analysis (ANOVA, Tukey test) was used to compare fluorescence intensities.
Main Results:
- The most effective combination for detecting tooth and bone was a 455 nm wavelength with an orange filter.
- Fluorescence intensity was highest in dental root, followed by enamel, and then bone.
- Biological materials demonstrated significantly higher fluorescence than inert materials.
Conclusions:
- The 455 nm wavelength and orange filter provide optimal detection of tooth and bone.
- Understanding differential fluorescence aids in forensic identification of fragmented remains.
- This technique can assist forensic experts in both field and laboratory settings.