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Updated: Sep 2, 2026

Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato
Published on: June 9, 2009
Absence of some truncated genes in the amphidiploid Nicotiana tabacum
1Institut de Biologie Moléculaire des Plantes du C.N.R.S., Strasbourg, France.
Abstract:
As an initial step towards understanding how a multigene family evolves after an interspecific hybridization and subsequent chromosomal doubling, genomic Southern blots of three related species were compared: Nicotiana tabacum (the progeny), and Nicotiana sylvestris and Nicotiana tomentosiformis (the progenitors). Genomic restriction fragments generated by two endonucleases were hybridized with a cDNA of the small subunit of ribulose bisphosphate carboxylase from N. sylvestris. The restriction pattern of the DNA of the progenitors revealed considerable polymorphism of restriction-fragment lengths. All the fragments in N. tabacum, except one, have a corresponding fragment in one of the progenitors. Some fragments present in the parents were absent from the progeny: they may correspond to truncated genes consisting of only part of the 3' portion of the gene.
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