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Optimized PCR-based Detection of Mycoplasma
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Optimized PCR-based Detection of Mycoplasma

Published on: June 20, 2011

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Routine testing of Mycoplasma genitalium and Trichomonas vaginalis

Marieke L M Jenniskens1, Jan H W Veerbeek1, Koen L Deurloo1

  • 1a Department of Obstetrics and Gynaecology , Diakonessenhuis , Utrecht , the Netherlands.

Infectious Diseases (London, England)
|March 18, 2017
PubMed
Abstract

Insights

Mycoplasma genitalium (MG) is more common than Neisseria gonorrhoeae (NG) and Trichomonas vaginalis (TV) in the Netherlands. These findings suggest that MG and TV should be considered for routine STI screening.

Area of Science:

  • Infectious Diseases
  • Microbiology
  • Public Health

Background:

  • Mycoplasma genitalium (MG) and Trichomonas vaginalis (TV) are common sexually transmitted infections (STIs).
  • Routine STI screening programs often exclude MG and TV, leading to a lack of epidemiological data.
  • This study addresses the knowledge gap regarding MG and TV prevalence in the general population.

Purpose of the Study:

  • To determine the prevalence of Mycoplasma genitalium (MG) and Trichomonas vaginalis (TV) in a regional Dutch population.
  • To provide data to inform decisions on including MG and TV in routine STI testing protocols.
  • To compare the prevalence of MG and TV with established STIs like Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG).

Main Methods:

  • A laboratory-developed RT-PCR assay was used for additional detection of MG and TV.
  • Samples submitted for routine STI testing (CT and NG) between February 2013 and August 2015 were retrospectively analyzed.
  • Samples were collected from both hospital and regional general practitioner settings.

Main Results:

  • Out of 5628 samples, 7.5% tested positive for at least one STI.
  • Mycoplasma genitalium (MG) was detected in 1.9% of samples, while Trichomonas vaginalis (TV) was found in 0.6%.
  • Chlamydia trachomatis (CT) prevalence was 5%, and Neisseria gonorrhoeae (NG) was 0.5%. CT and NG were more frequent in primary care and in men.

Conclusions:

  • Mycoplasma genitalium (MG) is more prevalent than Neisseria gonorrhoeae (NG) and Trichomonas vaginalis (TV) in the studied Dutch population.
  • Trichomonas vaginalis (TV) showed a similar prevalence to Neisseria gonorrhoeae (NG).
  • The study recommends considering the inclusion of MG and TV in routine STI screening protocols based on their prevalence.
Keywords:
Mycoplasma genitaliumSTITrichomonas vaginalis

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