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A clinical isolate of transposon Tn5 expressing streptomycin resistance in Escherichia coli
O Genilloud1, J Blázquez, P Mazodier
1Unidad de Genética Molecular, Hospital Ramón y Cajal, Madrid, Spain.
Journal of Bacteriology
|March 1, 1988
Abstract:
The central region of transposon Tn5 carries three antibiotic resistance markers: neo, ble, and str. The str gene codes for a phosphotransferase that inactivates streptomycin. This activity is phenotypically expressed in several gram-negative bacteria but not in Escherichia coli. We identified a Tn5 variant in E. coli clinical isolates that express streptomycin resistance. This transposon carries a 6-base-pair deletion within the str gene, near the 3' end. The same kind of mutation had been previously obtained experimentally from Tn5.