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Complete Genome Sequence of Streptococcus pyogenes emm14 JS95, a Necrotizing Fasciitis Strain Isolated in Israel

Jacqueline L Y Chee1, Miriam Ravins2, Emanuel Hanski2,3

  • 1Genome Institute of Singapore, Singapore.

Genome Announcements
|March 18, 2017
PubMed

Insights

We sequenced the genome of the Streptococcus pyogenes emm14 strain JS95, found in a necrotizing fasciitis patient. This strain contains the streptococcal invasion locus (sil), a key quorum-sensing system in S. pyogenes.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Streptococcus pyogenes is a significant human pathogen.
  • Necrotizing fasciitis is a severe invasive infection caused by S. pyogenes.
  • Understanding virulence factors is crucial for combating S. pyogenes infections.

Purpose of the Study:

  • To report the complete genome sequence of a specific S. pyogenes emm14 strain (JS95).
  • To identify key genetic elements, such as quorum-sensing systems, within this strain.
  • To contribute to the genomic understanding of S. pyogenes virulence.

Main Methods:

  • Whole-genome sequencing of Streptococcus pyogenes emm14 strain JS95.
  • Bioinformatic analysis to identify genes and genetic loci.
  • Comparative genomics to characterize virulence factors.

Main Results:

  • The complete genome sequence of S. pyogenes emm14 strain JS95 was obtained.
  • The streptococcal invasion locus (sil), a quorum-sensing system, was identified in strain JS95.
  • This represents the first characterization of the sil system in S. pyogenes.

Conclusions:

  • The genome sequencing provides a valuable resource for studying S. pyogenes virulence.
  • The presence of the sil locus in this invasive strain highlights its potential role in pathogenesis.
  • Further research on the sil system can inform therapeutic strategies against S. pyogenes infections.

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