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Published on: March 11, 2014
Multiplex polymerase chain reaction for the detection of high-risk-human papillomavirus types in formalin-fixed
Mini P Singh1, Nalini Gupta2, T Deepak1
1Department of Virology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Abstract:
Detecting high-risk-human papillomavirus (HPV) types has become an integral part of the cervical cancer screening programmes. This study aimed to develop a multiplex polymerase chain reaction (PCR) for identification of HPV types 16 and 18 along with the beta globin gene in formalin-fixed and paraffin-embedded cervical biopsy specimens. A total of 59 samples from patients with cervical abnormalities were tested. HPV 16 positivity was 50% in cervical cancers and 52.9% in cervical intraepithelial neoplasia. Our multiplex PCR protocol can be used as a simple and cost-effective tool for high-risk-HPV detection in cervical cancer screening programmes.
