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Chromosome replication in Escherichia coli induced by oversupply of DnaA
Abstract:
Overexpression of DnaA protein from a multicopy plasmid accompanied by a shift to 42 degrees C causes initiation of one extra round of replication in a dnaA+ strain grown in glycerol minimal medium. This extra round of replication does not lead to an extra cell division, such that cells contain twice the normal number of chromosomes.