Early grey crescent formation experimentally induced by cycloheximide in the axolotl oocyte

The effects of cycloheximide (CH) on grey crescent formation in artificially maturedAmbystoma mexicanum oocytes were determined. CH induced grey crescent formation after a few hours, especially after a 45° to 90° rotation from the vertical animal-vegetal axis. With low concentrations of CH (about 0.5 ng/oocyte), meiosis was still able to proceed normally to the stable second metaphase stage, but higher concentrations blocked it after 1st polar body extrusion and an interphasic nucleus appeared. Such effects were compared to those of inactone, an analogue of cycloheximide, which as a pure substance does not inhibit protein synthesis, but still contained a small amount of CH in the available samples. It is concluded that grey crescent formation can occur in non-activated oocytes. The effects of cycloheximide might be due to partial inhibition of protein synthesis and the presence of a proteinic inhibitor of the symmetry reaction in the normal oocyte is suggested.