The annulate lamellae of salamander oocytes: Morphological and functional aspects

The present study reports observations on the distribution, morphology and functional significance of annulate lamellae in the developing salamander oocyte. This organelle was found in the cytoplasm, both as individual elements and as a cluster made up of from two to approximately five lamellae organized into closely aligned stacks. Adjacent stacks of lamellae were found to be randomly oriented. Annulate lamellae are essentially identical in structure with the nuclear envelope. The terminations of lamellae were very frequently found to be expanded into sac-like structures. The outer surface of each sac is usually lined with ribosomes. The paired membranes in some of the lamellae exhibited dilations for varying distances from their terminal sacs. These dilated segments of the lamellae were also lined with ribosomes. The annulate lamellae were interpreted as being intermediate stages in the formation of rough-surfaced cisternae and would, therefore, represent structural precursors of a form of endoplasmic reticulum. This organelle may therefore represent a transient morphological response to a need for especially high concentrations of proteins.