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Updated: Mar 6, 2026

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
Published on: March 5, 2019
RNA Polymerase B levels during the cell cycle ofPhysarum polycephalum
Gérard Pierron1, Helmut W Sauer1
1Zoologisches Institut der Universität, Röntgenring 10, D-8700, Würzburg, Federal Republic of Germany.
Abstract:
Tritiated α-amanitin has been used as a specific and sensitive probe to estimate the number of RNA polymerase B molecules in isolated nuclei, chromatin and nucleoids, obtained from macroplasmodia ofPhysarum polycephalum. During mitosis (metaphase±10 min) there is at least 10-fold less RNA polymerase B than at all phases of the cell cycle, even if DNA replication has been blocked in vivo. It is concluded that many of the RNA polymerase B molecules leave the chromatin during decondensation of the chromosomes in telophase of the synchronous nuclear division ofPhysarum.
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