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Related Experiment Video

Updated: Mar 6, 2026

Visualizing Cytoskeleton-Dependent Trafficking of Lipid-Containing Organelles in Drosophila Embryos
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Dye-coupling in the early squid embryo.

Hans -Jürg Marthy1, Brian Dale2

  • 1Laboratoire Arago, UA 117 du CNRS, Universitè P. et M. Curie, F-66650, Banyuls-sur-mer, France.

Roux'S Archives of Developmental Biology : the Official Organ of the EDBO
|March 18, 2017
PubMed
Summary
This summary is machine-generated.

Squid embryos possess two cell communication pathways: one for small molecules (like gap junctions) and another for larger molecules (like cytoplasmic bridges). These pathways differ in their function during the cell cycle.

Keywords:
Dye-couplingGerm layersSquid embryos

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Area of Science:

  • Developmental Biology
  • Cell Biology
  • Embryology

Background:

  • Understanding cell-to-cell communication is crucial for embryonic development.
  • Early embryonic stages offer a model for studying fundamental cellular processes.

Purpose of the Study:

  • To investigate the nature and properties of cell-to-cell communication pathways in pre-organogenetic squid embryos.
  • To differentiate between pathways for small molecules and macromolecules.

Main Methods:

  • Microinjection of fluorescent dyes Lucifer Yellow-CH (M.Wt.450) and Fluorescein-isothiocyanate Dextran (M.Wt.9000 or 17,500) into squid embryonic cells.
  • Observation of dye spread to determine pathway permeability and distribution.

Main Results:

  • Lucifer Yellow (small molecule) spread extensively, crossing germ layers and yolk syncytium.
  • Fluorescein-isothiocyanate Dextran (large molecule) spread was limited, confined to adjacent cells within the same germ layer.
  • Two distinct pathways were identified: one for small molecules (ubiquitous, gap junction-like) and one for macromolecules (larger junction, cytoplasmic bridge-like).

Conclusions:

  • Squid embryos exhibit size-selective cell communication pathways.
  • The small molecule pathway is active throughout the cell cycle, while the macromolecule pathway is interphase-dependent and restricted during mitosis.