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Updated: Mar 6, 2026
A Rapid and Efficient Method to Dissect Pupal Wings of Drosophila Suitable for Immunodetections or PCR Assays
Published on: December 30, 2017
The cell biology of Drosophila wing metamorphosis in vitro
Martin J Milner1, Jonathan Muir1
1Department of Zoology and Marine Biology, University of St. Andrews, KY16 9TS, St. Andrews, Fife, Scotland.
Abstract:
We have examined the metamorphosis of the wing imaginal disc of Drosophila during culture in vitro in the continuous presence of 20-hydroxy ecdysone (0.1 μg/ ml). We find that the sequence of cellular changes in the wing blade during culture closely match those occurring in situ, involving two periods at which the dorsal and ventral surfaces are joined only by cell processes containing trans-alar microtubule arrays. Good pupal and imaginal cuticle secretion is found in this system.
