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Estrogen-dependent in vitro transcription from the vitellogenin promoter in liver nuclear extracts
B Corthésy1, R Hipskind, I Theulaz
1Institut de Biologie animale, Université de Lausanne, Switzerland.
Science (New York, N.Y.)
|March 4, 1988
Abstract:
One approach to analyzing the molecular mechanisms of gene expression in vivo is to reconstitute these events in cell-free systems in vitro. Although there is some evidence for tissue-specific transcription in vitro, transcriptionally active extracts that mimic a steroid hormone-dependent enhancement of transcription have not been described. In the study reported here, nuclear extracts of liver from the frog Xenopus laevis were capable of estrogen-dependent induction of a homologous vitellogenin promoter that contained the estrogen-responsive element.