Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rotavirus serotype-specific neutralizing activity in human milk.

L M Bell1, H F Clark, P A Offit

  • 1Wistar Institute of Anatomy and Biology, Children's Hospital, Philadelphia, PA 19104.

American Journal of Diseases of Children (1960)
|March 1, 1988
PubMed
Summary

Human breast milk contains rotavirus-neutralizing antibodies, with activity persisting long after birth. Breastfeeding duration correlates with higher antibody levels, offering potential infant protection.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

COVID-19 stressors, wellbeing and health behaviours: a cross-sectional study.

Journal of public health (Oxford, England)·2021
Same author

Site of PGF<sub>2α</sub> injection does not alter effectiveness of the Select Synch + controlled internal drug release and timed artificial insemination protocol.

Theriogenology·2016
Same author

Immunologic methods and correlates of protection.

Methods in molecular medicine·2011
Same author

Phosphorus metabolism of preschool children.

The Journal of nutrition·2010
Same author

The newer technic of intracapsular cataract extraction.

The Journal of the American Osteopathic Association·2010
Same author

Divergent regulation of hypothalamic neuropeptide Y and agouti-related protein by photoperiod in F344 rats with differential food intake and growth.

Journal of neuroendocrinology·2009

Area of Science:

  • Immunology
  • Pediatrics
  • Virology

Background:

  • Human breast milk is a crucial source of passive immunity for infants.
  • Rotavirus is a leading cause of severe diarrheal disease in infants worldwide.
  • Understanding the dynamics of rotavirus-specific antibodies in breast milk is essential for infant health.

Purpose of the Study:

  • To determine rotavirus serotype-specific neutralizing activity in human breast milk.
  • To investigate the correlation between socioeconomic status and antibody levels.
  • To assess the persistence and fluctuation of neutralizing activity postpartum.

Main Methods:

  • Plaque reduction neutralization assay (PRNT) was employed.
  • Enzyme-linked immunosorbent assay (ELISA) was used to detect rotavirus-specific antibodies (IgA and IgG).

Related Experiment Videos

  • Analysis included samples from mothers of varying socioeconomic backgrounds and longitudinal samples from five mothers.

    • Main Results:

    • The majority of breast milk samples exhibited rotavirus neutralizing activity.
    • Neutralizing activity and antibody levels were comparable across different socioeconomic groups.
    • Prevalence of neutralizing antibodies was high against Wa (77%), SA-11 (86%), and NCDV (75%) rotaviruses.
    • Rotavirus-specific IgA and IgG antibodies were detected in 35% and 55% of samples, respectively.
    • Activity fluctuated over time, with some mothers showing high levels up to 18 months postpartum.
    • Extended breastfeeding (≥6 months) was associated with higher milk neutralizing titers.

    Conclusions:

    • Human breast milk possesses significant rotavirus-specific neutralizing activity.
    • This activity can persist for extended periods postpartum, potentially offering prolonged infant protection.
    • Breastfeeding duration appears to positively influence the levels of these protective antibodies.