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Related Experiment Videos

Evaluation of three methods for typing herpes simplex virus.

F Salmerón1, P Pérez Breña, J M Rojas

  • 1Centro Nacional de Farmacobiología, Carretera de Majadahonda a Pozuelo, Madrid, Spain.

European Journal of Clinical Microbiology
|December 1, 1987
PubMed
Summary

Comparing herpes simplex virus typing methods, immunofluorescence assay showed differences between types 1 and 2. However, some antiviral drug-resistant mutants may be misidentified using this approach.

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Area of Science:

  • Virology
  • Microbiology
  • Infectious Diseases

Background:

  • Herpes simplex virus (HSV) typing is crucial for clinical management and epidemiological studies.
  • Accurate differentiation between HSV-1 and HSV-2 is essential for targeted antiviral therapy and understanding disease patterns.

Purpose of the Study:

  • To compare the efficacy of different methods for typing herpes simplex virus clinical isolates.
  • To evaluate the reliability of immunofluorescence assay and restriction endonuclease analysis in differentiating HSV-1 and HSV-2.
  • To assess the impact of antiviral drug resistance on viral typing.

Main Methods:

  • Immunofluorescence assay (IFA) using type-specific antibodies was performed on 111 clinical isolates and 7 resistant mutants.
  • Restriction endonuclease analysis was conducted on 29 of the clinical isolates.

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  • Antiviral sensitivity testing to (E)-5-(2-bromovinyl)-2'-deoxyuridine was performed.

    • Main Results:

    • Immunofluorescence assay demonstrated a discernible difference in the 50% inhibitory dose (ID50) between HSV-1 and HSV-2 isolates.
    • Some antiviral drug-resistant mutants exhibited characteristics that could lead to misidentification by the immunofluorescence assay.
    • Restriction endonuclease analysis provided complementary data for viral typing.

    Conclusions:

    • Immunofluorescence assay is a useful method for differentiating HSV-1 and HSV-2, but its accuracy may be compromised by drug-resistant strains.
    • Careful interpretation is needed when using immunofluorescence assay for typing, especially in the presence of antiviral resistance.
    • Further studies may be warranted to refine typing methods for drug-resistant HSV mutants.