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Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
Published on: August 16, 2018
Growth pattern and carbon allocation to volatile leaf terpenes under nitrogen-limiting conditions in Heterotheca
Charles A Mihaliak1, David E Lincoln1
1Department of Biology, University of South Carolina, 29208, Columbia, SC, USA.
Abstract:
Rosettes of Heterotheca subaxillaris were grown at four levels of nitrate. Individual leaf volatile mono- and sesquiterpene content, leaf nitrogen content, and root and shoot dry weight were measured on individual leaves every two weeks for 18 weeks. Rosettes with the highest nitrate availability had 2.2-fold greater leaf nitrogen levels compared to plants with the lowest availability. As nitrate availability became increasingly limited, carbon allocation to both volatile leaf terpenes and root growht increased. Leaf mono- and sesquiterpene content was greatest in the young leaves of individuals growing at the lowest nitrate availability conditions. Higher levels of carbon-based herbivore-deterring chemicals in nitrate-limited plants may increase net productivity through retention of nitrogen that would otherwise be lost to herbivory.
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