Nutrient loading of a forest soil : A manipulative approach using rooted experimental chambers
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Updated: Mar 6, 2026

An Optimized Rhizobox Protocol to Visualize Root Growth and Responsiveness to Localized Nutrients
Published on: October 22, 2018
T W Willison1, P R Splatt1, J M Anderson1
1Department of Biological Sciences, Hatherly Laboratory, University of Exeter, EX4 4PS, Exeter, Devon, England.
Abstract:
During July 1988 rooted and non-rooted experimental chambers were established in a Norway spruce (Picea abies. Karst) stand in south Devon U.K. Replicates were supplemented with ammonium and nitrate. The leachates were analysed to monitor the release of mineral-N species and cations over the 17-week experimental period. Ammonium treatments leached 300% more calcium and magnesium than controls. The onset of nitrification resulted in a decrease in sodium losses from ammonium treatments reflecting a decrease in the exchanging capacity of the soil solution. These results are discussed in relation to mineral ion leaching in soils subjected to increesed N-loading, and the ability of soils to buffer these perturbations.

