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Interaction of ceftriaxone with human polymorphonuclear neutrophil function.

M T Labro¹, C Babin-Chevaye, I Pochet

¹INSERM U. 294, Centre Hospitalo-Universitaire Xavier Bichat, Paris, France.

The Journal of Antimicrobial Chemotherapy

|December 1, 1987

Summary

Ceftriaxone does not directly enhance human neutrophil functions like killing bacteria or phagocytosis. The observed synergy in bacterial killing likely stems from ceftriaxone altering the bacteria, not the immune cells.

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Area of Science:

Immunology
Pharmacology
Microbiology

Background:

Ceftriaxone, a cephalosporin antibiotic, has demonstrated in vitro synergy with human neutrophils against certain bacteria.
Previous studies suggest a cooperative effect between ceftriaxone and leukocyte bactericidal activity.

Purpose of the Study:

To investigate the direct impact of ceftriaxone on human neutrophil functions.
To determine if ceftriaxone enhances neutrophil-mediated bacterial killing and phagocytosis.

Main Methods:

Assessed neutrophil chemotaxis and superoxide anion production in the presence of ceftriaxone.
Measured neutrophil chemiluminescence response and phagocytosis of Staphylococcus aureus and Klebsiella pneumoniae.
Evaluated the effect of ceftriaxone on isolated neutrophils and whole human blood stimulated with zymosan.

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Main Results:

Ceftriaxone did not affect neutrophil chemotaxis or superoxide anion production.
No enhancement of bacterial killing or phagocytosis was observed; phagocytosis was depressed with adherent neutrophils.
A paradoxical depressive effect on chemiluminescence was noted in whole blood with zymosan stimulation.

Conclusions:

The synergy between ceftriaxone and leukocytes for bacterial killing is not due to direct stimulation of neutrophil functions.
The observed synergy is likely attributed to ceftriaxone-induced alterations in bacterial susceptibility.