St₂-80: a new FISH marker for St genome and genome analysis in Triticeae

The St genome is one of the most fundamental genomes in Triticeae. Repetitive sequences are widely used to distinguish different genomes or species. The primary objectives of this study were to (i) screen a new sequence that could easily distinguish the chromosome of the St genome from those of other genomes by fluorescence in situ hybridization (FISH) and (ii) investigate the genome constitution of some species that remain uncertain and controversial. We used degenerated oligonucleotide primer PCR (Dop-PCR), Dot-blot, and FISH to screen for a new marker of the St genome and to test the efficiency of this marker in the detection of the St chromosome at different ploidy levels. Signals produced by a new FISH marker (denoted St₂-80) were present on the entire arm of chromosomes of the St genome, except in the centromeric region. On the contrary, St₂-80 signals were present in the terminal region of chromosomes of the E, H, P, and Y genomes. No signal was detected in the A and B genomes, and only weak signals were detected in the terminal region of chromosomes of the D genome. St₂-80 signals were obvious and stable in chromosomes of different genomes, whether diploid or polyploid. Therefore, St₂-80 is a potential and useful FISH marker that can be used to distinguish the St genome from those of other genomes in Triticeae.