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Lentinula edodes Genome Survey and Postharvest Transcriptome Analysis.

Yuichi Sakamoto1, Keiko Nakade2, Shiho Sato2

  • 1Iwate Biotechnology Research Center, Kitakami-shi, Iwate, Japan sakamoto@ibrc.or.jp.

Applied and Environmental Microbiology
|March 19, 2017
PubMed
Summary
This summary is machine-generated.

Researchers sequenced the genome of Lentinula edodes, identifying genes responsible for postharvest quality loss. This study provides targets for breeding mushroom strains with improved shelf life.

Keywords:
chitinfruiting bodyglucanlentinansenescence

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Area of Science:

  • Mycology
  • Genomics
  • Biochemistry

Background:

  • Lentinula edodes (shiitake mushroom) is a popular edible and medicinal fungus.
  • Postharvest issues like browning, softening, and nutrient degradation affect shiitake quality.
  • Understanding the genetic basis of these changes is crucial for improving shelf life.

Purpose of the Study:

  • To construct a de novo draft genome sequence of Lentinula edodes.
  • To identify genes involved in postharvest quality deterioration.
  • To provide targets for breeding shiitake mushrooms with enhanced longevity.

Main Methods:

  • De novo genome assembly using paired-end, mate-paired, and PacBio long reads.
  • Gene prediction using Augustus and RNA-seq data.
  • Transcriptome analysis (SuperSAGE) to identify differentially expressed genes postharvest.

Main Results:

  • A draft genome assembly of 1,951 contigs was generated, with 12,959 predicted genes.
  • Lentinula edodes lacks lignin peroxidase.
  • Upregulation of cell wall-degrading enzymes (e.g., glucanases, chitinases) and transcription factors postharvest suggests rapid autolysis.

Conclusions:

  • A rapid fruiting body autolysis system exists in Lentinula edodes.
  • Identified genes offer targets for breeding shiitake strains with extended freshness.
  • The genome assembly data will aid in constructing a complete chromosome map for future breeding efforts.