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Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE
Published on: April 21, 2019
2D-Electrophoresis and Immunoblotting in Food Allergy
Galina Grishina1, Luda Bardina2, Alexander Grishin2
1Elliot and Roslyn Jaffe Food Allergy Institute, Division of Allergy and Immunology, Department of Pediatrics, Icahn School of Medicine at Mount Sinai, 1 Gustave L Levy Place, New York, 10029, NY, USA. galina.grishina@mssm.edu.
Abstract:
Two-dimensional polyacrylamide gel electrophoresis (2DE) and Western immunoblotting have proven to be invaluable and fundamental techniques for comprehensive characterization of food allergens. Here, we describe and discuss detailed protocols used in our studies on identification of allergenic proteins in shrimp, sesame, hazelnut, and pistachio.
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