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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
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Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE
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Protein Microarray-Based IgE Immunoassay for Allergy Diagnosis.

Nuzul N Jambari1, XiaoWei Wang2, Marcos Alcocer2

  • 1Department of Food Sciences, Faculty of Food Sciences and Technology, University of Putra Malaysia, UPM Serdang, 43400, Serdang, Selangor, Malaysia. noorahya@upm.edu.my.

Methods in Molecular Biology (Clifton, N.J.)
|March 19, 2017
PubMed
Summary
This summary is machine-generated.

This study details a novel protein microarray for detecting specific antibodies in horse serum. The flexible platform enables diverse immunoassay analyses, advancing diagnostic capabilities.

Keywords:
Component-resolved diagnosticsHorse seraIgE-binding assayMicroarray-based allergy testingPartial Least Squares Discriminant Analysis (PLSDA)Protein microarrayVariable influence on the projection (VIP)

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Area of Science:

  • Biotechnology
  • Immunology
  • Analytical Chemistry

Background:

  • Protein microarrays are miniaturized, solid-phase immunoassays for detecting specific binding interactions.
  • They utilize immobilized proteins on a slide to capture antibodies from biological samples like serum.
  • Fluorescent labeling and advanced algorithms are key for quantitative analysis.

Purpose of the Study:

  • To describe the development and application of an in-house complex protein microarray.
  • To demonstrate its use in probing horse serum for specific antibodies.
  • To highlight the platform's flexibility for various immunoassay analyses.

Main Methods:

  • Production of a complex protein microarray with diverse extracts and pure proteins.
  • Probing the microarray with antibodies from horse serum samples.
  • Detection using fluorophore-conjugated antibodies and advanced image processing algorithms.

Main Results:

  • Successful detection of specific antibodies in horse serum using the custom protein microarray.
  • Quantitative analysis of binding interactions through fluorescent signal measurement.
  • Demonstration of the microarray's capacity for multiple, specific IgE immunoassay analyses.

Conclusions:

  • The developed in-house protein microarray is a versatile tool for antibody detection.
  • Its flexible design supports a wide range of specific immunoassay applications.
  • This technology offers potential for enhanced diagnostic capabilities in equine health.