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Related Experiment Video

Updated: Mar 6, 2026

A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent

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Basophil Degranulation Assay.

Madhan Masilamani^1,2, Mohanapriya Kamalakannan³, Hugh A Sampson^4,5

¹Division of Allergy and Immunology, Department of Pediatrics, The Jaffe Food Allergy Institute, Icahn School of Medicine at Mount Sinai, Icahn Building, Room 11-23B, Box 1089, New York, NY, 10029, USA. madhan.masilamani@mssm.edu.

Methods in Molecular Biology (Clifton, N.J.)

|March 19, 2017

Summary

The basophil degranulation assay measures CD63 expression to diagnose food allergies and track immunotherapy progress. This method quantizes a key marker released during basophil activation.

Keywords:

Basophils CD63 Degranulation Flow cytometry In vitro test

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Area of Science:

Immunology
Allergy diagnostics

Background:

Basophil degranulation assays are increasingly vital for diagnosing food allergies.
These assays help evaluate the effectiveness of immunotherapy in allergy patients.

Purpose of the Study:

To detail the methodology for performing the basophil degranulation assay.
To explain the role of CD63 as a marker in this diagnostic process.

Main Methods:

The assay identifies and quantifies CD63 molecule expression on the basophil cell membrane.
CD63 is a marker for multivesicular bodies exposed during degranulation.

Main Results:

The assay provides a quantifiable measure of basophil activation.
Successful execution allows for accurate allergy diagnosis and immunotherapy monitoring.

Conclusions:

The basophil degranulation assay is a reliable method for allergy assessment.
Understanding the methodology is key to its effective application in clinical settings.