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DTNB-Based Quantification of In Vitro Enzymatic N-Terminal Acetyltransferase Activity
Håvard Foyn1, Paul R Thompson2, Thomas Arnesen3,4
1Department of Molecular Biology, University of Bergen, Thormøhlensgate 55, N-5020, Bergen, Norway.
Methods in Molecular Biology (Clifton, N.J.)
|March 19, 2017
Abstract:
We here describe a quick and easy method to quantitatively measure in vitro acetylation activity of not only N-terminal acetyltransferase (NAT) enzymes, but acetyltransferases using acetyl-coenzyme A as an acetyl donor in general.
