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Antigen based detection of cystic echinococcosis in buffaloes using ELISA and Dot-EIA
A Sangaran1, S T Bino Sundar1, Bhaskaran Ravi Latha1
1Department of Veterinary Parasitology, Madras Veterinary College, Tamilnadu Veterinary and Animal Sciences University, Chennai, 600 007 India.
Abstract:
Cystic echinococcosis is caused by the larval stage of the dog tapeworm, Echinococcus granulosus. The disease is recognized as one of the world's major zoonoses affecting human beings and domestic animals apart from its economic and public health importance. Development of the cysts in the intermediate host such as buffaloes occurs in the lungs, liver and other organs. In this study, detection of circulating antigen in the diagnosis of cystic echinococcosis in buffaloes was done using enzyme linked immunosorbent assay and Dot-Enzyme immunoassay (Dot-EIA). The sensitivity and specificity were determined as 89 and 92 % respectively, whereas those of Dot-EIA were determined as 94 and 96 %.
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