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Related Experiment Videos

Does the beta-adrenergic receptor function as a reovirus receptor?

A H Choi1, P W Lee

  • 1Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, Alberta, Canada.

Virology
|March 1, 1988
PubMed
Summary

This study investigated if beta-adrenergic receptors are universal signals for reovirus binding. Reovirus and beta-adrenergic receptors on A431 cells were found to be distinct, suggesting different cellular recognition mechanisms.

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Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • A type 3 reovirus receptor on mouse R1.1 cells shares structural similarities with mammalian beta-adrenergic receptors.
  • Human epidermoid carcinoma A431 cells possess numerous functional beta-adrenergic receptors and are susceptible to reovirus infection.

Purpose of the Study:

  • To determine if beta-adrenergic receptors serve as universal recognition sites for reovirus binding.
  • To investigate the functional relationship between reovirus and beta-adrenergic receptors in A431 cells.

Main Methods:

  • Assessing reovirus binding and internalization in A431 cells.
  • Evaluating cellular adenylate cyclase activity in response to reovirus and beta-adrenergic agonists/antagonists.
  • Investigating the effect of beta-adrenergic receptor sequestration on reovirus binding.

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Main Results:

  • Reovirus binding and internalization in A431 cells did not activate cellular adenylate cyclase.
  • Reovirus presence did not affect cellular responses to the beta-adrenergic agonist (-)-isoproterenol or antagonist [3H]CGP-12,177 binding.
  • Sequestration of beta-adrenergic receptors did not alter reovirus binding, and vice versa.

Conclusions:

  • Reovirus and beta-adrenergic receptors on A431 cells are distinct entities.
  • Beta-adrenergic receptors are not universal recognition signals for type 3 reovirus binding.