Microscopic characterization of Pseudomonas Aeruginosa confines separate from clinical cases by testing RAPD-PCR...

Area of Science:

• Medical Microbiology
• Infectious Diseases
• Molecular Biology

Background:

• Pseudomonas Aeruginosa is a major opportunistic pathogen, particularly affecting immune-compromised individuals like burn victims.
• Carbapenems are crucial for treating resistant strains, but understanding resistance mechanisms and transmission is vital for controlling nosocomial infections.

Purpose of the Study:

• To investigate the antibiotic sensitivity profile of Pseudomonas Aeruginosa isolates from burn patients.
• To determine the genetic patterns of these isolates using the RAPD-PCR technique.
• To correlate genetic diversity with antibiotic resistance for improved infection control strategies.

Main Methods:

• Antibiotic susceptibility testing (Antibiogram) was performed on Pseudomonas Aeruginosa isolates.
• Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) was employed for genetic fingerprinting.
• Specific primers (272, 277, 287) were used to analyze genetic polymorphism.

Main Results:

• Gentamicin showed 50% sensitivity, while Nalidixic Acid, Erythromycin, and Cefotaxime exhibited 90% resistance.
• RAPD-PCR with primers 272, 277, and 287 revealed 18, 15, and 11 distinct genetic patterns (algorithms), respectively.
• The study demonstrated significant genetic diversity among the Pseudomonas Aeruginosa isolates.

Conclusions:

• Antibiotic resistance is a significant concern in Pseudomonas Aeruginosa infections among burn patients.
• RAPD-PCR is an effective tool for bacterial genotyping, essential for epidemiological studies and understanding resistance transmission.
• These findings support the development of targeted infection control measures based on genetic profiling.