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Microscopic characterization of Pseudomonas Aeruginosa confines separate from clinical cases by testing RAPD-PCR
Z Mahmmudi1, A Emami2, A A Gorzin3
1Kazeroun Branch, Islamic Azad University, Kazeroon, Iran.
Abstract:
Pseudomonas Aeruginosa is one of the leading opportunistic infectious agents especially in immune-suppressed patients such like burn victims. Carbapenems like Imipenem (IMP) and Meropenem (MEM) are the choice antimicrobial drugs, which used in the treatment of Penicillin and Aminoglycoside-resistant Pseudomonas Aeruginosa isolates. Based on the importance of the detection of infectious source and their resistance transfer algorithm have a main effect on the control of nosocomial infections this study designed for to consider the antibiotic sensitivity and the genetic pattern of Pseudomonas Aeruginosa isolate in burned patients hospitalized in Ghotbeddin Shirazi Hospital with RAPD-PCR technique. According to the Antibiogram results, the most sensitivity was related to the Gentamicin with 50% while the most resistant related to the Nalidixic Acid, Erythromycin, and Cefotaxime with 90% resistant to all. With RAPD-PCR technique used primers 272, 277 and 287 were detected 18, 15, and 11 algorithms respectively. RAPD-PCR is a genotyping method with a high efficiency and good for the study of bacterial epidemiology and polymorphism.
Insights
This study analyzed antibiotic resistance and genetic patterns in Pseudomonas Aeruginosa from burn patients. RAPD-PCR identified distinct genetic profiles, aiding in understanding infection spread and control.
Area of Science:
- Medical Microbiology
- Infectious Diseases
- Molecular Biology
Background:
- Pseudomonas Aeruginosa is a major opportunistic pathogen, particularly affecting immune-compromised individuals like burn victims.
- Carbapenems are crucial for treating resistant strains, but understanding resistance mechanisms and transmission is vital for controlling nosocomial infections.
Purpose of the Study:
- To investigate the antibiotic sensitivity profile of Pseudomonas Aeruginosa isolates from burn patients.
- To determine the genetic patterns of these isolates using the RAPD-PCR technique.
- To correlate genetic diversity with antibiotic resistance for improved infection control strategies.
Main Methods:
- Antibiotic susceptibility testing (Antibiogram) was performed on Pseudomonas Aeruginosa isolates.
- Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) was employed for genetic fingerprinting.
- Specific primers (272, 277, 287) were used to analyze genetic polymorphism.
Main Results:
- Gentamicin showed 50% sensitivity, while Nalidixic Acid, Erythromycin, and Cefotaxime exhibited 90% resistance.
- RAPD-PCR with primers 272, 277, and 287 revealed 18, 15, and 11 distinct genetic patterns (algorithms), respectively.
- The study demonstrated significant genetic diversity among the Pseudomonas Aeruginosa isolates.
Conclusions:
- Antibiotic resistance is a significant concern in Pseudomonas Aeruginosa infections among burn patients.
- RAPD-PCR is an effective tool for bacterial genotyping, essential for epidemiological studies and understanding resistance transmission.
- These findings support the development of targeted infection control measures based on genetic profiling.
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