Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

ANF in experimental congestive heart failure.

M Cantin1, G Thibault, J F Ding

  • 1Laboratory of Pathobiology, Clinical Research Institute of Montreal, Québec, Canada.

The American Journal of Pathology
|March 1, 1988
PubMed
Summary

Plasma atrial natriuretic factor (ANF) levels increase in heart failure, originating from both atrial and ventricular cardiocytes. This study correlates ANF changes with cellular alterations in cardiomyopathic hamsters.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

"I want nothing to do with hospitals anymore": challenges, met and unmet needs of people experiencing homelessness with acquired brain injury.

Brain injury·2026
Same author

"Overnight, everything changed": life courses and turning points toward homelessness among people with acquired brain injury.

Brain injury·2026
Same author

Oral oligofructose challenge reduces expression of glucose transport-1 and 5'-adenosine monophosphate-activated protein kinase in lamellar wall of Holstein heifer claw.

Research in veterinary science·2021
Same author

Effects of oxycodone hydrochloride on immune function and biochemical indexes in puerperas undergoing cesarean section.

Journal of biological regulators and homeostatic agents·2019
Same author

Effects of submaximal and supramaximal interval training on determinants of endurance performance in endurance athletes.

Scandinavian journal of medicine & science in sports·2016
Same author

[N-methyl-D-aspartate receptor antibody encephalitis: value of immunomodulatory therapy].

Archives de pediatrie : organe officiel de la Societe francaise de pediatrie·2014

Area of Science:

  • Cardiology
  • Cell Biology
  • Pathophysiology

Background:

  • Congestive heart failure (CHF) is a complex condition characterized by cardiac dysfunction.
  • Atrial natriuretic factor (ANF) plays a crucial role in cardiovascular homeostasis.
  • Understanding ANF regulation in heart disease is vital for therapeutic development.

Purpose of the Study:

  • To investigate the changes in plasma and cardiac levels of immunoreactive (IR) atrial natriuretic factor (ANF) throughout the lifespan of cardiomyopathic hamsters.
  • To correlate these changes with cellular alterations in the secretory apparatus of atrial and ventricular cardiocytes.
  • To elucidate the mechanisms of ANF synthesis and release in the context of developing heart failure.

Main Methods:

  • Measurement of plasma and cardiac IR-ANF levels in cardiomyopathic hamsters.

Related Experiment Videos

  • Immunohistochemical, ultrastructural, and immunocytochemical analyses of atrial and ventricular cardiocytes.
  • Assessment of secretory granule content and Golgi complex morphology using immunogold techniques.

    • Main Results:

    • Plasma IR-ANF increased in early disease stages, peaked in moderate heart failure, and remained elevated.
    • Cardiac ANF decreased in atria but increased significantly in ventricles, particularly the left ventricle.
    • Atrial cardiocytes showed increased Golgi size and decreased secretory granules, suggesting a dissociation between synthesis and release.
    • Ventricular cardiocytes exhibited increased Golgi size and the presence of secretory granules, indicating increased synthesis and potential release pathways.

    Conclusions:

    • Elevated plasma IR-ANF in CHF may result from secretion by both atrial and ventricular cardiocytes.
    • Atrial cardiocytes demonstrate a maximal release despite reduced granule content.
    • Ventricular cardiocytes show enhanced ANF synthesis and dual release pathways (constitutive and regulated).