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Updated: Mar 6, 2026

Supercritical Nitrogen Processing for the Purification of Reactive Porous Materials
Published on: May 15, 2015
A nano-delivery system for bioactive ingredients using supercritical carbon dioxide and its release behaviors
Wenbei Situ1, Xianliang Song1, Shucan Luo1
1College of Food Science, South China Agricultural University, Guangzhou 510640, China.
Abstract:
For the purpose of ensuring the bioavailability of bioactive ingredients, a nano-delivery system with low toxicity was developed using supercritical carbon dioxide (SC-CO2). Compared to thin-film hydration (TFH), obtaining nano-scale liposomes is easier using SC-CO2. The characteristic of these liposomes was also demonstrated by the analysis of particle size and morphology. An in vitro release study showed that liposomes produced using SC-CO2 were resistant to low pH in simulated gastric conditions. In a simulated intestinal environment, enteric solubility of these liposomes was enhanced, which are important properties for controlled releasing bioactive ingredient. Furthermore, SC-CO2-produced liposomes had a higher storage stability than those produced using TFH. Analysis of the organic solvent residue in the liposomes by gas chromatography-mass spectrometry (GC-MS) indicated that SC-CO2-produced liposomes had lower toxicity than those produced by TFH. A chemical free nano-delivery system using SC-CO2 has been revealed for storage and controlled release of bioactive ingredients.
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