Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

2025 Korean Guidelines for Cardiopulmonary Resuscitation: Part 9. Neonatal resuscitation.

Clinical and experimental emergency medicine·2026

Same author

Degradation-controlled synchronization of HIF-2α and MEK inhibition using self-sealed porous silicon nanoparticles to reprogram tumor immunogenicity.

Acta biomaterialia·2026

Same author

Structural predictors of mechanical instability after posterolateral elbow dislocation: a magnetic resonance imaging-based threshold model.

Journal of shoulder and elbow surgery·2026

Same author

Site-Specific Calibration of Low-Cost Particulate Matter (PM) 2.5 Monitors in the United States: A Comparison of Industrial and Non-Industrial Communities.

Aerosol and air quality research·2026

Same author

Integrated Size-Selective Cell Purification and Electroporation for Genetic Manipulation of Primary Cells.

Micromachines·2026

Same author

Volatile organic compounds exposure and all health outcomes: An umbrella review and evidence map.

Environmental research·2026

Same journal

Therapeutic potential of crude protein extracts from two Egyptian freshwater snails Lanistes carinatus and Bellamya unicolor.

Scientific reports·2026

Same journal

Microbial contamination of donor corneas and post-keratoplasty endophthalmitis: a comparison between Japanese and U.S. eye banks using cold storage.

Scientific reports·2026

Same journal

Prevalence and contributing factors of virological non-suppression among adult patients on first-line antiretroviral therapy in tertiary hospitals in Ethiopia.

Scientific reports·2026

Same journal

An in vitro comparison of color stability between alkasite and different restorative materials in various staining solutions.

Scientific reports·2026

Same journal

Toward accessible mRNA LNP formulation: systematic evaluation of mixing strategies and key parameters.

Scientific reports·2026

Same journal

A network analysis of personality traits, mentalizing, and psychological health in Chinese college students.

Scientific reports·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 6, 2026

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

Published on: August 7, 2014

Microscale Symmetrical Electroporator Array as a Versatile Molecular Delivery System.

Mengxing Ouyang¹, Winfield Hill¹, Jung Hyun Lee²

¹Rowland Institute at Harvard University, 100 Edwin H. Land Blvd., Cambridge, MA 02142, USA.

Scientific Reports

|March 21, 2017

Summary

This summary is machine-generated.

This study presents a novel vortex-assisted electroporation system for precise, low-voltage intracellular delivery of multiple molecules into cells. The technology offers dosage control and real-time optimization for diverse cell types, advancing therapeutic development.

More Related Videos

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays MEA

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays MEA

Published on: September 13, 2012

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

Published on: January 7, 2022

Related Experiment Videos

Last Updated: Mar 6, 2026

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

Published on: August 7, 2014

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays MEA

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays MEA

Published on: September 13, 2012

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

Published on: January 7, 2022

Area of Science:

Biotechnology
Cell Biology
Bioengineering

Background:

Intracellular delivery of exogenous molecules is crucial for developing novel therapeutic strategies.
Existing methods for molecular delivery often face limitations in control, efficiency, and versatility.

Purpose of the Study:

To develop a versatile on-chip vortex-assisted electroporation system for sequential, dosage-controlled intracellular delivery of multiple molecules.
To enable low-voltage electroporation and real-time optimization for various cell types.

Main Methods:

Development of an on-chip vortex-assisted electroporation system with micro-patterned planar electrodes.
Integration with microfluidic technology and real-time process visualization.
Demonstration of utility through RNA interference assays.

Main Results:

Achieved sequential intracellular delivery of multiple molecules into various cell types at low voltage.
Demonstrated dosage control and on-chip optimization of electroporation parameters.
Successfully utilized the system for RNA interference assays, validating its research applicability.

Conclusions:

The developed system offers a powerful tool for precise, single-cell level co-administration of multiple molecules with controlled dosages.
This technology has broad potential applications in advancing therapeutic strategies and biological research.
The system facilitates efficient and controlled intracellular delivery, overcoming limitations of current methods.