How does ytterbium chloride interact with DMPC bilayers? A computational and experimental study
Miguel A Gonzalez1, Hanna M G Barriga1, Joanna L Richens2
1Department of Chemistry, Imperial College London, SW7 2AZ, London, UK. m.gonzalez12@imperial.ac.uk fbresme@imperial.ac.uk hanna.barriga04@imperial.ac.uk.
Lanthanide ions like Ytterbium (Yb3+) can bind to lipid bilayers without disrupting them. These interactions increase bilayer rigidity, impacting Nuclear Magnetic Resonance (NMR) experiment interpretations.
Area of Science:
- Biophysics
- Membrane Biophysics
- Computational Biophysics
Background:
- Lanthanide salts are widely used in Nuclear Magnetic Resonance (NMR) studies of lipid-protein systems and model membranes.
- Understanding molecular-level lipid-lanthanide interactions is crucial for interpreting experimental data.
Purpose of the Study:
- To investigate lanthanide-bilayer interactions at a molecular level.
- To elucidate the effects of Ytterbium (Yb3+) on DMPC lipid bilayer properties.
Main Methods:
- Molecular dynamics computer simulations.
- Fluorescence electrostatic potential experiments.
- Nuclear Magnetic Resonance (NMR) spectroscopy.
Main Results:
- Yb3+ ions adsorb to DMPC bilayers without structural disruption.
- Strong Yb3+-lipid binding occurs, decreasing area per lipid and increasing chain ordering and bilayer thickness.
- Bilayer rigidity significantly increases (doubles the area compressibility modulus).
Conclusions:
- Yb3+ ions modify lipid bilayer properties, enhancing rigidity.
- These lanthanide-induced changes must be considered when interpreting NMR data from lipid-protein systems.
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