Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mechanism of Angiogenesis

Mechanism of Angiogenesis

Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

Development of Blood Vessels

Development of Blood Vessels

The development of the vascular system in a fetus is a complex and intricate process that begins as early as 15 to 16 days post-conception. This process starts outside the embryo, specifically in the mesoderm of the yolk sac, chorion, and connecting stalk. Approximately two days later, the formation of blood vessels occurs within the embryo itself.
The initial formation of this system is facilitated by the small amount of yolk present in the ovum and yolk sac. Blood vessels originate from...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Engineering the blood-brain barrier on chip: advances in preclinical drug penetration and disease modeling.

Advanced drug delivery reviews·2026

Same author

Self-localized ultrafast pencil beam for volumetric multiphoton imaging.

Nature methods·2026

Same author

Quantitative comparison of methods for widespread delivery of small molecules across the blood-brain barrier.

Communications biology·2026

Same author

ETV2 Mediated Differentiation of Human Pluripotent Stem Cells Results in Functional Endothelial Cells for Engineering Advanced Vascularized Microphysiological Models.

Advanced healthcare materials·2026

Same author

Vascular STING activation facilitates NK cell anti-tumor immunity in small cell lung cancer.

Cancer cell·2026

Same author

miR126-mediated alteration of vascular integrity in Rett syndrome.

Molecular psychiatry·2026

Same journal

Industry updates in advanced therapy medicinal products and regenerative medicine - May 2026.

Regenerative medicine·2026

Same journal

Ethical, legal, and social issues associated with human fetal tissue research in Japan.

Regenerative medicine·2026

Same journal

The future of hematopoietic stem cell and stem cell gene therapy for metabolic diseases.

Regenerative medicine·2026

Same journal

Exploring gene therapy for developmental and epileptic encephalopathies (DEEs): possibilities or promises?

Regenerative medicine·2026

Same journal

Industry updates in advanced therapy medicinal products and regenerative medicine - April 2026.

Regenerative medicine·2026

Same journal

Optimizing strategies in tendon tissue engineering through effective scaffold design: overview of recent advancements.

Regenerative medicine·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 6, 2026

Procedure for the Development of Multi-depth Circular Cross-sectional Endothelialized Microchannels-on-a-chip

Procedure for the Development of Multi-depth Circular Cross-sectional Endothelialized Microchannels-on-a-chip

Published on: October 21, 2013

Advances in on-chip vascularization.

Kristina Haase¹, Roger D Kamm^1,2,3

¹Department of Mechanical Engineering, MIT, Cambridge, MA, USA.

Regenerative Medicine

|March 21, 2017

Summary

This summary is machine-generated.

Microfluidics enables advanced in vitro models for studying blood vessels and organ development. These microfluidic systems offer precise control for applications in drug screening and regenerative medicine.

Keywords:

angiogenesis microfluidics microvasculature organ-on-a-chip permeability regenerative medicine tissue-engineering vasculogenesis

More Related Videos

Author Spotlight: Improving Reproducibility in Vascular Organoids Using ROCK Inhibitors and Microwell Confinement

Author Spotlight: Improving Reproducibility in Vascular Organoids Using ROCK Inhibitors and Microwell Confinement

Published on: December 13, 2024

Author Spotlight: Studying hiPSC-Derived Endothelial Cells Cultured Under Fluidic-Mediated Mechanical Stimulation

Author Spotlight: Studying hiPSC-Derived Endothelial Cells Cultured Under Fluidic-Mediated Mechanical Stimulation

Published on: July 28, 2023

Related Experiment Videos

Last Updated: Mar 6, 2026

Procedure for the Development of Multi-depth Circular Cross-sectional Endothelialized Microchannels-on-a-chip

Procedure for the Development of Multi-depth Circular Cross-sectional Endothelialized Microchannels-on-a-chip

Published on: October 21, 2013

Author Spotlight: Improving Reproducibility in Vascular Organoids Using ROCK Inhibitors and Microwell Confinement

Author Spotlight: Improving Reproducibility in Vascular Organoids Using ROCK Inhibitors and Microwell Confinement

Published on: December 13, 2024

Author Spotlight: Studying hiPSC-Derived Endothelial Cells Cultured Under Fluidic-Mediated Mechanical Stimulation

Author Spotlight: Studying hiPSC-Derived Endothelial Cells Cultured Under Fluidic-Mediated Mechanical Stimulation

Published on: July 28, 2023

Area of Science:

Biomedical Engineering
Microfluidics
Tissue Engineering

Background:

Microfluidics is crucial for studying microvasculature, organ-on-chip models, and microtissue engineering.
Microfluidic systems precisely control geometrical, biochemical, and mechanical factors to simulate in vivo conditions.

Purpose of the Study:

To review the generation and application of in vitro vascular networks using microfluidic technology.
To highlight the potential of microfluidics in drug screening and regenerative medicine.

Main Methods:

Generation of in vitro vascular networks via endothelial-lined patterned channels or self-assembled networks.
Application of microfluidic techniques for rapid perfusion in tissue and organ-mimicking models.
Integration with tuneable hydrogels for enhanced functionality.

Main Results:

Microfluidic approaches effectively mimic in vivo conditions, controlling shear and interstitial flow.
Both patterned and self-assembled vascular networks are suitable for studying angiogenesis, vasculogenesis, and cancer metastasis.
Successful in vivo implantation of microfluidic-generated vascular networks has been demonstrated.

Conclusions:

Microfluidics provides a powerful platform for creating complex in vitro vascular models.
This technology holds significant promise for accelerating drug discovery and advancing regenerative medicine through prevascularized tissue development.