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Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
Published on: June 30, 2018
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Co-localization of proteins with defined sequential order and controlled stoichiometric ratio on magnetic
Yi Yue1, Yang-Yang Lu1, Mei Li1
1College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China. fanlh@mail.buct.edu.cn and Beijing Key Laboratory of Bioprocess, Beijing 100029, China.
Nanoscale
|March 21, 2017
Abstract:
Co-immobilization of enzymes used in cascade reactions is important for improving the overall catalytic efficiency. In this work, we employed scaffoldins as a bridge and succeeded in a highly-ordered co-localization of multiple proteins on magnetic nanoparticles with a loading capacity of ∼0.831 μmol g-1 supports.

