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Updated: Mar 6, 2026

Chicken Recombinant Limbs Assay to Understand Morphogenesis, Patterning, and Early Steps in Cell Differentiation
Published on: January 12, 2022
The two domain hypothesis of limb prepattern and its relevance to congenital limb anomalies
Hirotaka Tao1, Yasuhiko Kawakami2, Chi-Chung Hui1,3
1Program in Developmental and Stem Cell Biology, Research Institute, The Hospital for Sick Children, Toronto, Canada.
Abstract:
Functional annotation of mutations that cause human limb anomalies is enabled by basic developmental studies. In this study, we focus on the prepatterning stage of limb development and discuss a recent model that proposes anterior and posterior domains of the early limb bud generate two halves of the future skeleton. By comparing phenotypes in humans with those in model organisms, we evaluate whether this prepatterning concept helps to annotate human disease alleles. WIREs Dev Biol 2017, 6:e270. doi: 10.1002/wdev.270 For further resources related to this article, please visit the WIREs website.
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