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A new mutation for multiple drug resistance and modified plasma membrane ATPase activity in Schizosaccharomyces pombe
S Ulaszewski1, A Coddington, A Goffeau
1Laboratoire d'Enzymologie, Université de Louvain, Louvain-La-Neuve, Belgium.
Abstract:
The mutant JV66 was selected from the wild type strain of S. pombe 972h- ade7-413 by its ability to grow on solid rich medium containing 200 micrograms Dio-9/ml. The single nuclear mutation, designated pma1 gives resistance towards diguanidines and several other positively charged compounds. The pma1 mutation also decreases plasma membrane ATPase activity and confers resistance of ATPase to vanadate. The pma1 locus is localized on chromosome I at 5.3 map units from cyh1-C7 and at about 20.7 map units from the centromere. This new mutation is genetically and phenotypically different from the mutation cyh3 and cyh4 previously described (Johnston and Coddington 1983).
Insights
A new mutation, pma1, in fission yeast confers resistance to positively charged compounds like Dio-9. This mutation affects plasma membrane ATPase activity and vanadate resistance.
Area of Science:
- Molecular biology
- Genetics
- Biochemistry
Background:
- The study investigates mutations affecting cellular transport and enzyme activity in Schizosaccharomyces pombe.
- Previous research identified mutations cyh3 and cyh4 with similar resistance phenotypes.
Purpose of the Study:
- To characterize a novel mutation, pma1, conferring resistance to specific compounds.
- To understand the impact of pma1 on plasma membrane ATPase activity and vanadate sensitivity.
Main Methods:
- Selection of mutant JV66 from wild-type S. pombe 972h- ade7-413 on Dio-9 containing medium.
- Genetic analysis to identify and map the pma1 locus on chromosome I.
- Biochemical assays to assess plasma membrane ATPase activity and vanadate resistance.
Main Results:
- The pma1 mutation confers resistance to diguanidines and other positively charged compounds.
- pma1 leads to decreased plasma membrane ATPase activity.
- The mutation also confers resistance of the ATPase to vanadate.
- The pma1 locus was mapped to chromosome I, distinct from previously described mutations.
Conclusions:
- The pma1 mutation represents a new genetic locus in S. pombe with unique characteristics.
- pma1 provides insights into the regulation of plasma membrane ATPase and transport mechanisms.