Related Experiment Video

Updated: Mar 6, 2026

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
08:58

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Published on: March 3, 2023

8.2K

Moraxella Species as Potential Sources of MCR-Like Polymyxin Resistance Determinants

Nicolas Kieffer1,2, Patrice Nordmann1,2,3,4, Laurent Poirel5,2,3

  • 1Emerging Antibiotic Resistance, Medical and Molecular Microbiology Unit, Department of Medicine, University of Fribourg, Fribourg, Switzerland.

Antimicrobial Agents and Chemotherapy
|March 22, 2017
PubMed

Insights

The MCR-1/2 gene, conferring resistance to polymyxins, was found in Moraxella species. These bacteria, often animal pathogens, may act as reservoirs for these resistance genes.

Area of Science:

  • Microbiology
  • Genetics
  • Antimicrobial Resistance

Background:

  • Plasmid-mediated resistance to polymyxins, driven by MCR-1/2 determinants, is a growing global concern within Enterobacteriaceae.
  • Polymyxins are critical last-resort antibiotics for treating multidrug-resistant Gram-negative bacterial infections.

Purpose of the Study:

  • To investigate the presence of mcr-like genes in Moraxella species.
  • To identify potential animal reservoirs for polymyxin resistance genes.

Main Methods:

  • Polymerase chain reaction (PCR) techniques were employed to screen Moraxella isolates.
  • Gene cloning strategies were utilized to confirm the presence and function of mcr-like genes.

Main Results:

  • mcr-like genes were successfully identified in several Moraxella species.
  • Moraxella isolates were confirmed to carry genes conferring polymyxin resistance.

Conclusions:

  • Moraxella species, known animal and potential human pathogens, represent a significant reservoir for mcr-like genes.
  • The findings highlight the need to monitor Moraxella for polymyxin resistance mechanisms to prevent further spread.
Keywords:
MCRanimalplasmidpolymyxinreservoirresistance

More Related Videos

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus09:03

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

12.4K
Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

11.5K

Related Concept Videos

Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
1.7K
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
792
Bacterial Phylum Tenericutes01:24

Bacterial Phylum Tenericutes

The phylum Tenericutes, which includes the single class Mollicutes, comprises bacteria that lack cell walls. The term "Mollicutes" derives from the Latin word mollis, meaning "soft." These organisms are among the smallest known and are commonly referred to as mycoplasmas due to the prominence of the genus Mycoplasma, which includes well-known human pathogens. Despite their inability to stain gram-positively (a result of their lack of cell walls), mycoplasmas are phylogenetically related to the...
554
Antibiotic Selection00:57

Antibiotic Selection

Overview
61.6K