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Related Concept Videos

Role of Skin in Vitamin D Synthesis01:23

Role of Skin in Vitamin D Synthesis

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The skin plays a crucial role in the synthesis of vitamin D, a vital nutrient for various physiological processes in the body. Vitamin D is unique because it can be synthesized in the skin through a series of chemical reactions triggered by exposure to ultraviolet B (UVB) radiation from sunlight.
The solar UV B rays (290-315 nm) are absorbed by the skin, and 7-dehydrocholesterol (provitamin D3) photolyzes it to previtamin D3, which undergoes a rapid transformation to vitamin...
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Bioavailability Study Design: Healthy Subjects Versus Patients01:15

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Bioavailability studies are essential for evaluating a drug's therapeutic efficacy and understanding its absorption patterns under various physiological conditions. Conducting such studies on target patient populations provides more relevant data by simulating real-world disease states. However, practical challenges often necessitate the use of young, healthy adult volunteers as study subjects.Patients may exhibit altered drug absorption patterns due to the effects of the disease itself,...
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A Method of Trigonometric Modelling of Seasonal Variation Demonstrated with Multiple Sclerosis Relapse Data
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Standardizing 25-hydroxyvitamin D data from the HunMen cohort.

E Jakab1, E Kalina1, Z Petho2

  • 1Department of Laboratory Medicine, University of Debrecen, Nagyerdei Blvd 98, Debrecen, H-4032, Hungary.

Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA
|March 22, 2017
PubMed
Summary
This summary is machine-generated.

Standardizing 25-hydroxyvitamin D (25OHD) measurements is crucial. This study presents a practical method using NIST values to correct lab results, improving accuracy and reducing hypovitaminosis D prevalence.

Keywords:
25-Hydroxyvitamin DDEQASHunMenStandardization

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Area of Science:

  • Clinical Chemistry
  • Biochemistry
  • Laboratory Medicine

Background:

  • Accurate measurement of 25-hydroxyvitamin D (25OHD) is essential for clinical diagnosis and management.
  • Current 25OHD assays lack standardization, leading to significant inter-laboratory variability and potential misdiagnosis.
  • External Quality Assessment Schemes (EQAS) provide valuable data for assessing and improving assay performance.

Purpose of the Study:

  • To develop and validate a practical method for standardizing 25-hydroxyvitamin D (25OHD) values in routine diagnostic laboratories.
  • To correct measured 25OHD values using linear regression against National Institute of Standards and Technology (NIST) target values.
  • To assess the impact of standardization on the prevalence of hypovitaminosis D.

Main Methods:

  • A linear regression model was developed using data from the Vitamin D External Quality Assessment Scheme (DEQAS).
  • The model utilized the relationship between laboratory-measured total 25OHD and NIST total target values (TV) for DEQAS survey samples.
  • The developed standardization approach was applied to 206 samples from the HunMen cohort measured by chemiluminescence immunoassay (CLIA).

Main Results:

  • Standardization using the NIST-DEQAS linear regression model significantly adjusted the mean total 25OHD in the HunMen cohort from 53 to 62 nmol/L.
  • The prevalence of hypovitaminosis D (<75 nmol/L) in the cohort decreased substantially from 84% to 72% after standardization.
  • The method demonstrated a practical approach for routine diagnostic laboratories to address non-standardized 25OHD values.

Conclusions:

  • A simple and practical method for standardizing 25-hydroxyvitamin D (25OHD) measurements is proposed.
  • Correction using NIST target values via DEQAS provides a viable solution to the challenge of non-standardized 25OHD values.
  • This approach can improve the accuracy of 25OHD reporting and clinical decision-making.