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Related Concept Videos

Ligand Binding Sites02:40

Ligand Binding Sites

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Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
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The Equilibrium Binding Constant and Binding Strength02:18

The Equilibrium Binding Constant and Binding Strength

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The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:
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Related Experiment Video

Updated: Mar 6, 2026

Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer
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Current Trends in Ligand Binding Real-Time Measurement Technologies.

Stephanie Fraser1, Judy Y Shih2, Mark Ware3

  • 1Pfizer, Eastern Point Rd, Groton, Connecticut, 06340, USA. Stephanie.Fraser@pfizer.com.

The AAPS Journal
|March 22, 2017
PubMed
Summary

Recent ligand binding assay (LBA) technologies offer real-time measurements for improved receptor/ligand interaction analysis and faster clinical treatment decisions through point-of-care (POC) applications.

Keywords:
emergent technology reviewlateral flow immunoassayligand binding assayreal-time measurementsurface plasmon resonance

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biotechnology

Background:

  • Ligand binding assays (LBAs) are crucial for analyzing molecular interactions.
  • Traditional LBAs have limitations in real-time data acquisition and clinical application.
  • Recent technological advancements aim to overcome these limitations.

Purpose of the Study:

  • To review recent advances in real-time LBA technologies.
  • To highlight technologies enhancing experimental analysis of binding interactions.
  • To showcase point-of-care (POC) LBA technologies for clinical decision-making.

Main Methods:

  • Review of novel and adapted LBA platforms focusing on real-time measurement.
  • Analysis of improvements in throughput, multiplexing, and sensitivity.
  • Evaluation of clinical utility of POC LBA technologies.

Main Results:

  • New LBA platforms and optimized existing ones offer real-time binding event measurement.
  • Significant improvements in assay throughput, multiplexing capabilities, and sensitivity have been achieved.
  • POC LBA technologies provide immediate data for rapid clinical decisions.

Conclusions:

  • Real-time LBA technologies enhance the experimental interrogation of molecular binding.
  • Advancements in LBA platforms offer increased value for both research and clinical settings.
  • POC LBA innovations facilitate faster and more informed medical treatment decisions.