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Modulation of protein kinase C activity by NaF in bone marrow derived macrophages

S Hauschildt1, W Hirt, W Bessler

  • 1Institut für Immunbiologie, Universität Freiburg, FRG.

FEBS Letters
|March 28, 1988
PubMed

Insights

Sodium fluoride (NaF) stimulates macrophages, increasing inositol phosphates and diacylglycerol (DAG) release. This suggests NaF activates protein kinase C via DAG, potentially involving G-proteins in macrophage signaling.

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Macrophages play crucial roles in immune responses.
  • Cell signaling pathways regulate macrophage activation.
  • Phosphatidylinositol signaling is vital for cellular processes.

Purpose of the Study:

  • To investigate the effect of sodium fluoride (NaF) on murine bone marrow derived macrophages.
  • To elucidate the signaling pathways involved in NaF-induced macrophage activation.
  • To determine the role of diacylglycerol (DAG) and protein kinase C (PKC) in NaF-stimulated macrophages.

Main Methods:

  • Murine bone marrow derived macrophages were stimulated with NaF.
  • Cells were prelabeled with [1-14C]oleate and [3H]inositol.
  • Production of inositol phosphates and release of 1,2-[14C]diacylglycerol (DAG) were measured.
  • Protein kinase C (PKC) activation was assessed.

Main Results:

  • NaF stimulation increased inositol phosphate production.
  • NaF induced the release of 1,2-[14C]diacylglycerol (DAG).
  • NaF activated protein kinase C (PKC).

Conclusions:

  • Bone marrow derived macrophages possess a NaF-sensitive phosphatidyl-4,5-bisphosphate phospholipase C activity.
  • This activity may be modulated by G-proteins.
  • NaF-induced DAG release likely mediates PKC activation in macrophages.

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