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DissolvIt: An In Vitro Method for Simulating the Dissolution and Absorption of Inhaled Dry Powder Drugs in the Lungs
Per Gerde1,2, Maria Malmlöf1,2, Lina Havsborn1
11 Inhalation Sciences Sweden AB , Novum, Sweden .
Assay and Drug Development Technologies
|March 22, 2017
Summary
A new in vitro method, DissolvIt, accurately simulates inhaled drug dissolution and absorption in the lungs, mimicking real systemic pharmacokinetic data for drugs like budesonide.
Area of Science:
- Pharmacology
- Drug Delivery Systems
- In Vitro Modeling
Background:
- In vitro methods are crucial for predicting in vivo drug behavior.
- Simulating lung's air-blood barrier dissolution and absorption presents challenges.
- Existing models may not fully replicate systemic pharmacokinetic profiles.
Purpose of the Study:
- To develop an in vitro method (DissolvIt) for simulating inhaled dry powder drug dissolution and absorption.
- To ensure the developed method mimics systemic pharmacokinetic data.
- To evaluate the performance of the DissolvIt method.
Main Methods:
- Developed DissolvIt, a multi-component system simulating the air-blood barrier.
- Utilized PreciseInhale® for aerosolization and deposition of drug particles.
- Employed optical microscopy for dissolution observation and LC-MS/MS for quantitation.
- Measured drug concentration in perfusate and remaining drug in the barrier.
Main Results:
- DissolvIt successfully simulated particle dissolution upon contact with mucus simulant.
- Drug solute diffused through the barrier and was absorbed into the perfusate.
- Budesonide and fluticasone propionate exhibited distinct pharmacokinetic dissolution/absorption profiles.
- The method generated quantifiable drug concentration and absorption data.
Conclusions:
- DissolvIt effectively simulates lung drug dissolution and absorption processes.
- The developed in vitro method successfully mimics in vivo pharmacokinetic profiles and parameters.
- DissolvIt serves as a valuable tool for predicting inhaled drug performance.
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