Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromosome Replication02:31

Chromosome Replication

11.0K
Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
11.0K
Replication in Eukaryotes01:29

Replication in Eukaryotes

18.3K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
18.3K
Replication in Eukaryotes02:31

Replication in Eukaryotes

206.6K
Overview
206.6K
Nucleosome Remodeling02:54

Nucleosome Remodeling

11.5K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
11.5K
The DNA Replication Fork01:02

The DNA Replication Fork

41.8K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
41.8K
The DNA Replication Fork01:02

The DNA Replication Fork

19.0K
19.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comprehensive profiling of chromatin occupancy dynamics through the cell cycle.

Nucleic acids research·2026
Same author

Genome-wide nucleosome and transcription factor responses to genetic perturbations reveal chromatin-mediated mechanisms of transcriptional regulation.

Genome research·2025
Same author

CFDP1 is required for histone variant H2A.Z deposition by the human SRCAP chromatin remodeling complex.

bioRxiv : the preprint server for biology·2025
Same author

An Orc6 tether mediates ORC binding-site switching during replication origin licensing.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Dynamic Conformations of Chromatin Remodeler ISWI during Nucleosome Sliding Revealed by Hydrogen-Deuterium Exchange Coupled to Mass Spectrometry.

Biochemistry·2025
Same author

DNA mutagenesis driven by transcription factor competition with mismatch repair.

Cell·2025
Same journal

The exquisite mechanics of a tsetse bite.

eLife·2026
Same journal

Distinct involvements of the subthalamic nucleus subpopulations in reward-biased decision-making in monkeys.

eLife·2026
Same journal

Pink1-mediated mitophagy in the endothelium releases proteins encoded by mitochondrial DNA and activates neutrophil responses during inflammation.

eLife·2026
Same journal

Restraint of melanoma progression by cells in the local skin environment.

eLife·2026
Same journal

Brawn before bite in endemic Asian eutherian mammals after the end-Cretaceous extinction.

eLife·2026
Same journal

Experimental evolution to thermal stress indicates climate resilience in a cosmopolitan arthropod.

eLife·2026
See all related articles

Related Experiment Video

Updated: Mar 6, 2026

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

1.1K

Nucleosomes influence multiple steps during replication initiation.

Ishara F Azmi1, Shinya Watanabe2, Michael F Maloney1

  • 1Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, United States.

Elife
|March 22, 2017
PubMed
Summary
This summary is machine-generated.

Chromatin

Keywords:
DNA ReplicationEukaryoticHelicaseS. cerevisiaebiochemistrychromatin remodeling enzymechromosomesgenesnucleosomeorigin licensing

More Related Videos

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

4.2K
Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

8.9K

Related Experiment Videos

Last Updated: Mar 6, 2026

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

1.1K
Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

4.2K
Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

8.9K

Area of Science:

  • Molecular Biology
  • Epigenetics
  • DNA Replication

Background:

  • Chromatin structure influences eukaryotic DNA replication, but mechanisms remain unclear.
  • Understanding chromatin's role in replication origin control is crucial.

Purpose of the Study:

  • To investigate how nucleosomes impact DNA replication initiation.
  • To elucidate the mechanistic roles of chromatin remodeling enzymes in origin licensing and activation.

Main Methods:

  • Utilized reconstituted nucleosomal DNA replication assays.
  • Employed various chromatin-remodeling enzymes (CREs) to create distinct nucleosomal landscapes on origin DNA.
  • Performed origin licensing and helicase activation assays.

Main Results:

  • Nucleosomal organization directly affects origin licensing and helicase activation.
  • Local nucleosome positioning enhances origin specificity and modulates helicase loading by influencing ORC binding.
  • SWI/SNF- and RSC-remodeled nucleosomes allowed origin licensing but reduced helicase activation.
  • Specific CREs could rescue replication if added before helicase activation.

Conclusions:

  • Nucleosomes play distinct mechanistic roles in modulating origin licensing and helicase activation.
  • A permissive chromatin state during origin licensing is essential for efficient origin activation.
  • Provides insights into chromatin-based regulation of DNA replication initiation.