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Temporary Single-Cell Coating for Bioprocessing with a Cationic Polymer.

Ricardo D C Ribeiro1, Deepali Pal, David Jamieson

  • 1School of Mechanical and Systems Engineering, Newcastle University , Newcastle Upon Tyne NE1 7RU, U.K.

ACS Applied Materials & Interfaces
|March 22, 2017
PubMed
Summary

A new speckled coating method using poly(l-lysine) (PLL) temporarily coats cells, preventing aggregation for easier manipulation. Cells regain normal function after metabolizing the coating, offering a fast bioprocessing solution.

Keywords:
bioprintingbioprocessingcellular uptakecoatingpolycationsingle cellstemporary

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Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Biotechnology

Background:

  • Temporary single-cell coating is crucial for cell processing and manipulation.
  • Preventing cell attachment and agglomeration is key before re-establishing normal cell function.

Purpose of the Study:

  • To describe a speckled coating method using poly(l-lysine) (PLL) for temporary cell surface modification.
  • To investigate the effects of this coating on cell morphology, internalization, metabolization, and aggregation prevention.
  • To assess the feasibility of this method for bioprocessing and bioprinting applications.

Main Methods:

  • Inducing cell surface electrostatic charges using a polycation, poly(l-lysine) (PLL).
  • Applying a speckled coating method to three cell types: two bone cancer lines and fibroblasts.
  • Analyzing coating morphology, polymer internalization and metabolization, and cellular aggregation prevention.

Main Results:

  • Polymer concentration is a critical factor influencing both capsule morphology and cell health.
  • The PLL speckled coating effectively prevents cellular aggregations.
  • Cells exhibit phenotypically normal behavior after ingesting and metabolizing the polymer within 1-2 hours.

Conclusions:

  • The described speckled coating method offers a temporary, effective solution for single-cell manipulation in bioprocessing.
  • This approach provides a fast and efficient alternative for cell handling.
  • Preliminary results suggest potential applications in reliable bioprinting.