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IsoSel: Protein Isoform Selector for phylogenetic reconstructions.
Héloïse Philippon1, Alexia Souvane1, Céline Brochier-Armanet1
1Univ. Lyon, Université Claude Bernard Lyon 1, CNRS, Laboratoire de Biométrie et Biologie Evolutive, UMR 5558, F-69622, Villeurbanne, France.
Plos One
|March 22, 2017
Summary
Selecting the correct protein isoform is crucial for accurate molecular phylogenies. IsoSel is a new tool that outperforms existing methods for selecting protein isoforms, improving phylogenetic reconstruction reliability.
Area of Science:
- Genomics
- Bioinformatics
- Evolutionary Biology
Background:
- Molecular phylogenies rely on high-quality datasets for reliability.
- Eukaryotic phylogenetic analyses require selecting one protein isoform per locus to prevent redundancy bias.
Purpose of the Study:
- To introduce IsoSel, a novel computational tool for selecting protein isoforms in phylogenetic reconstruction.
- To offer an improved alternative to the common practice of selecting the longest protein isoforms.
Main Methods:
- IsoSel automates the selection of appropriate protein isoforms from genomic data.
- The tool's performance was benchmarked against existing methods, including the longest isoform approach and Guidance.
Main Results:
- IsoSel demonstrates superior performance compared to the longest isoform selection method.
- IsoSel outperforms Guidance, the only other available tool for this purpose.
Conclusions:
- IsoSel provides a more reliable method for protein isoform selection in phylogenetic studies.
- This tool enhances the accuracy of molecular phylogenies by addressing data redundancy issues.
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