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Updated: Mar 5, 2026

Analyzing Mitochondrial Transport and Morphology in Human Induced Pluripotent Stem Cell-Derived Neurons in Hereditary Spastic Paraplegia
Published on: February 9, 2020
Assessment of Mitophagy in iPS Cell-Derived Neurons
Kei-Ichi Ishikawa1, Akihiro Yamaguchi2, Hideyuki Okano3
1Department of Neurology, Juntendo University School of Medicine, Bunkyo, Tokyo, 113-8421, Japan.
Abstract:
Aberrant mitochondrial function is associated with many neurological diseases. Mitophagy is a key mechanism for the elimination of damaged mitochondria and maintenance of mitochondrial homeostasis. Induced pluripotent stem (iPS) cell technologies developed over the last decade have allowed us to analyze functions of the human neuron. Here we describe an efficient induction method from human iPS cells to neurons, followed by an image-based mitophagy assay.
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